Research Assistant Primer & Targeted Sequencing Panel Designer
HITS Solutions
نشرت في 24 ديسبمر 25
أرسل لي وظائف مثل هذه
الخبرة
1 - 2 سنوات
التعليم
بكالوريوس في التكنولوجيا/ الهندسة(التكنولوجيا الحيوية)
الجنسية
أي جنسية
جنس
غير مذكور
عدد الشواغر
1 عدد الشواغر
الوصف الوظيفي
الأدوار والمسؤوليات
- Design highly specific and uniform primers and multiplex PCR amplicon panels for targeted resequencing of cancer genes, hotspot mutations, fusions, CNVs, and other variants of interest.
- Perform in silico validation of primers/probes using tools such as Primer3, NCBI Primer-BLAST, BLAST, UCSC In-Silico PCR, Primer-BLAST, mfold/UNAfold, OligoAnalyzer, AutoDimerv1, Multiple Primer Analyzer, etc.
- Assess and minimize primer-dimer formation, off-target amplification, GC bias, and coverage imbalances in multiplex reactions.
- Design and maintain custom target BED files, manifest files, and panel metadata compatible with major NGS pipelines.
- Collaborate with wet-lab scientists to troubleshoot failed targets and iteratively improve panel performance (coverage, uniformity, sensitivity for low-VAF mutations).
- (Optional but valued) Design qPCR/RT-qPCR primers and TaqMan/Molecular Beacon probes for validation or orthogonal confirmation of mutations.
- Document all designs, parameters, and performance metrics in a clear, reproducible manner (version-controlled repositories preferred).
- Stay up to date with new tools (e.g., Primer Pooler, Pane Lapp, Open Primer, Amplicons, Panel Design, etc.) and publications on primer/design best practices.
- Bachelor s or master s degree in Molecular Biology, Genetics, Bioinformatics, Biotechnology, or a related field.
- At least 1 2 years of hands-on experience Designing Primers and/or Targeted Sequencing Panels (academic or industry).
- Strong understanding of NGS library preparation Chemistries (amplicon-based and Hybridization Capture).
- Proficiency with Primer Design Software and in Silico Evaluation Tools (Primer3Plus, NCBI tools, UCSC Genome Browser, Ensemble VEP, IGV, etc.).
- Solid knowledge of Human Genome Annotation, Repeat Masking, Pseudogenes, Paralogs, and Common Pitfalls in Mutation Detection Assays.
- Experience with Multiplex PCR Optimization and Uniformity Metrics (>90 95% on-target, 0.2 Mean Coverage Uniformity).
- Basic scripting ability (Python, Perl, Bash, or R) for automating design and analysis workflows is highly desirable.
- Excellent attention to detail, organizational skills, and ability to manage multiple targets/projects simultaneously.
الملف الشخصي المطلوب للمرشحين
- Bachelor s or master s degree in Molecular Biology, Genetics, Bioinformatics, Biotechnology, or a related field.
- At least 1 2 years of hands-on experience Designing Primers and/or Targeted Sequencing Panels (academic or industry).
- Strong understanding of NGS library preparation Chemistries (amplicon-based and Hybridization Capture).
- Proficiency with Primer Design Software and in Silico Evaluation Tools (Primer3Plus, NCBI tools, UCSC Genome Browser, Ensemble VEP, IGV, etc.).
- Solid knowledge of Human Genome Annotation, Repeat Masking, Pseudogenes, Paralogs, and Common Pitfalls in Mutation Detection Assays.
- Experience with Multiplex PCR Optimization and Uniformity Metrics (>90 95% on-target, 0.2 Mean Coverage Uniformity).
- Basic scripting ability (Python, Perl, Bash, or R) for automating design and analysis workflows is highly desirable.
- Excellent attention to detail, organizational skills, and ability to manage multiple targets/projects simultaneously.
القطاع المهني للشركة
- تكنولوجيا المعلومات - خدمات البرمجيات
المجال الوظيفي / القسم
- البحث والتطوير
الكلمات الرئيسية
- Research Assistant Primer & Targeted Sequencing Panel Designer
تنويه: نوكري غلف هو مجرد منصة لجمع الباحثين عن عمل وأصحاب العمل معا. وينصح المتقدمون بالبحث في حسن نية صاحب العمل المحتمل بشكل مستقل. نحن لا نؤيد أي طلبات لدفع الأموال وننصح بشدة ضد تبادل المعلومات الشخصية أو المصرفية ذات الصلة. نوصي أيضا زيارة نصائح أمنية للمزيد من المعلومات. إذا كنت تشك في أي احتيال أو سوء تصرف ، راسلنا عبر البريد الإلكتروني abuse@naukrigulf.com